ABSTRACT
Endometriosis (EMS) is one of the most prevalent causes for female infertility. Herein, we investigated the effect of the repaglinide (RG), L-carnitine (LC), and bone marrow mesenchymal stem cell-conditioned medium (BMSC-CM) supplementation during in vitro maturation (IVM) on the quality, maturation, and fertilization rates, as well as embryonic quality and development of oocytes derived from normal and EMS mouse model. Immature oocytes were collected from two groups of normal and EMS-induced female NMRI mice at 6-8 weeks of age. Oocytes were cultured in IVM medium unsupplemented (control group), or supplemented with 1 M RG, 0.3 and 0.6 mg/mL LC, and 25 and 50% BMSC-CM. After 24 h of oocyte incubation, IVM rate and antioxidant status were assessed. Subsequently, the rates of fertilization, cleavage, blastulation, and embryonic development were assessed. Our results demonstrated that supplementation of IVM medium with LC and BMSC-CM, especially 50% BMSC-CM, significantly enhanced IVM and fertilization rates, and markedly improved blastocyst development and total blastocyst cell numbers in EMS-induced mice compared to the control group (53.28±0.24 vs 18.09±0.10%). Additionally, LC and BMSC-CM were able to significantly modulate EMS-induced nitro-oxidative stress by boosting total antioxidant capacity (TAC) and mitigating nitric oxide (NO) levels. Collectively, LC and BMSC-CM supplementation improved oocyte quality and IVM rates, pre-implantation developmental competence of oocytes after in vitro fertilization, and enhanced total blastocyst cell numbers probably by attenuating nitro-oxidative stress and accelerating nuclear maturation of oocytes. These outcomes may provide novel approaches to refining the IVM conditions that can advance the efficiency of assisted reproductive technologies in infertile couples.
ABSTRACT
@#During pregnancy, Toxoplasma gondii can be transmitted from mother to foetus and trigger a primary infection that may be symptomatic. It is important to distinguish between recently acquired and past infections to ensure proper treatment to minimize irreversible foetal injury. We used PCR of the B1 gene to evaluate the accuracy of T. gondii IgG antibody avidity testing in discriminating recent from past infection. In a cross-sectional study, T. gondii IgG and IgM antibodies were detected by enzyme linked fluorescence assay (ELFA) in 2120 serum samples from pregnant women referred to Karaj medical laboratories, February 2013 through March 2015 with 40 samples found positive. IgM-positive samples were evaluated by IgG avidity testing and PCR to amplify the B1 gene. Avidity studies indicated 20 samples with high IgG avidity, 15 with low IgG avidity, and five showing borderline values. The B1 gene was amplified in the borderline samples, with nine of the 15 showing low avidity. The B1 gene was not amplified in the high avidity sera. Our findings suggest that IgG avidity alone may not be sufficient to discriminate recent from past T. gondii infection and should not be used as the sole confirmatory test in pregnant women with IgG and IgM T. gondii antibodies. IgG avidity testing in combination with PCR may be more reliable for distinguishing between high- and low-risk infection and decrease the frequency of unnecessary treatment of pregnant women.
ABSTRACT
Aim: Although one-fourth of the pituitary tumors are inactive and silent, increased total volume of the pituitary gland exerts pressure on sella turcica and corrodes the clinoid processes, resulting in several problems. Therefore, determination of the prevalence of the concealed pituitary tumors is of major concern among clinical practitioners. This study was performed to determine the prevalence of these tumors in cadavers referred to the Iranian Legal Medicine Organization (Kahrizak, Tehran). Materials and Methods: This cross-sectional descriptive study was performed between June 2007 and February 2008. A total of 485 cadavers were selected by simple random sampling method. Pituitary glands were removed and then horizontally cut and four slides were prepared from each of them, stained by hematoxylin and eosin, and evaluated by a light microscope. For statistical analysis, SPSS software (version 16), Mann-Whitney U test, Kolmogorov-Smirnov nonparametric test (K-S test), and Chi-square test were used. Results: Of the 485 investigated cadavers, 365 (75.3%) were males with an average age of 42 ± 20.5 years and 120 (24.7%) were females with an average age of 44 ± 22 years. A total of 61 (12.6%) had concealed pituitary masses. No statistically significant difference was found between the mean age, sex, and body mass index (BMI) of the cadavers with and without concealed tumors (P=0.380 P=0.450, and P=0.884, respectively). Conclusions: In the present study, the prevalence of the concealed pituitary adenomas was 12.6%. There was no correlation between age, sex, and BMI and the prevalence of the concealed masses.
Subject(s)
Adult , Cadaver , Cross-Sectional Studies , Female , Histocytochemistry , Humans , Iran/epidemiology , Male , Microscopy , Middle Aged , Pituitary Gland/pathology , Pituitary Neoplasms/epidemiology , PrevalenceABSTRACT
Background & objectives: Crimean-Congo hemorrhagic fever (CCHF) virus is a tick-borne member of the genus Nairovirus, family Bunyaviridae. CCHFV has been isolated from at least 31 different tick species. The virus is transmitted through the bite of an infected tick, or by direct contact with CCHFV-infected patients or the products of infected livestock. This study was undertaken to study the genetic relationship and distribution of CCHFV in the tick population of Hamadan province of Iran. Method: In this study, RT-PCR has been used for detection of the CCHFV genome. Results: This genome was detected in 19.2% of the ticks collected from livestock of different regions of the Hamadan province in western Iran. The infected species belonged to Hyalomma detritum, H. anatolicum, Rhipicephalus sanguineus and Argas reflexus. With one exception, genetic analysis of the virus genome isolates showed high sequence identity to each other. Even though they clustered in the same group with the strain circulating in Iran, they had a closer relationship to the Matin strain. Interpretation & conclusion: Vector control programs should be applied for reducing population density of potential tick vectors in this province. Further surveys are indicated in this region to provide a better view of the distribution and epidemiology of the virus.
ABSTRACT
Background & objectives: Kala-azar is the visceral and most severe form of leishmaniasis that leads to death if untreated. The causative agents of visceral leishmaniasis (VL) are members of Leishmania (L.) donovani complex which includes L. chagasi and L. infantum. Genome sequences have raised the question whether L. chagasi and L. infantum are synonymous or different. This question has important implications for clinical and epidemiological studies, evaluation of vaccines and drugs, and disease control. LCR1 is an immunogenic molecule discovered from L. chagasi with potential as a component of a Leishmania subunit vaccine. If this protein has potentials for being used in a vaccine or diagnostic testing, there should be little variability in this molecule between L. infantum isolates from diverse geographic regions. The aim of this study was to determine whether lcr1 of an Iranian strain of L. infantum was identical to lcr1 of both L. infantum strain from a different geographic region (Spain) and that of an L. chagasi isolate from Brazil. Methods: L. infantum isolated from an Iranian kala-azar patient was studied. Lcr1 from this isolate was PCR amplified, cloned, and studied by restriction digest analysis and sequencing. Results: The sequences of lcr1 of the Iranian L. infantum were completely identical at nucleotide level to lcr1 sequences of both the Spanish L. infantum and the Brazilian L. chagasi strains. Conclusion: Complete conservation of the DNA sequence encoding for LCR1 molecule between geographically distinct Leishmania species adds credibility to the potential for LCR1 as a component of a subunit vaccine and diagnostic test for kala-azar.
ABSTRACT
Background: Nasal polyposis is the benign protrusion of soft tissue into the nasal cavity; with multifactorial origin. This study is designed to examine the suggested role of IgE and CD and CD lymphocytes in the pathogenesis of 4 8 nasal polyposis. Method: Blood samples were taken from 32 patients with chronic polypoid sinusitis and 32 controls. CD and CD 4 8 total lymphocyte count were determined by flow cytometry and the level of serum IgE was measured by ELISA. Nasal discharge samples were also collected for determining IgE level in both patients and controls during surgery. Results: In 68.8of patients a history of allergy was present. The level of nasal discharge IgE was significantly higher (p0.001) in patients compared to controls; but the difference between serum IgE levels was not significant (p 0.05). CD concentration and blood lymphocytes were 8 significantly higher (p0.001) in the patients group; while CD concentration was significantly lower (p0.001) in 4 them. Finally; CD /CD ratio was significantly lower 4 8 (p0.001) in the patients group. Conclusion: This study suggests that a change in the amount of CD and CD lymphocytes and an increased 4 8 level of local IgE contribute to nasal polyposis; but the results should be confirmed in more extensive studies including cytokine analyses. Such increasing insights in the patho physiology of nasal polyposis open perspectives for new pharmacological treatment options; with immunologic factors as potential targets.of patients a history of allergy was present. The level of nasal discharge IgE was significantly higher (p0.001) in patients compared to controls; but the difference between serum IgE levels was not significant (p 0.05). CD concentration and blood lymphocytes were 8 significantly higher (p0.001) in the patients group; while CD concentration was significantly lower (p0.001) in 4 them. Finally; CD /CD ratio was significantly lower 4 8 (p0.001) in the patients group. Conclusion: This study suggests that a change in the amount of CD and CD lymphocytes and an increased 4 8 level of local IgE contribute to nasal polyposis; but the results should be confirmed in more extensive studies including cytokine analyses. Such increasing insights in the patho physiology of nasal polyposis open perspectives for new pharmacological treatment options; with immunologic factors as potential targets
Subject(s)
Nasal Cavity , Sinusitis/immunologyABSTRACT
Disability-Adjusted Life Years [DALY] summarizes the fatal and nonfatal outcomes of diseases and injuries in one number and gives a quantitative assessment of the health of a population. Estimating the burden of diseases and injuries in Iran in terms of DALY both nationwide and in 6 provinces. We used slightly modified versions of the methods developed by the World Health Organization for estimating the burden of premature mortality, disability, and the DALY. The DALY rate per 100,000 was 21572 and 62% of this was life lost due to premature mortality; the remaining 38% was due to disability from diseases and injuries. Fifty-eight percent of the total DALYs had been lost due to non-communicable diseases, 28% due to external causes [injuries], and 14% due to communicable, maternal/ perinatal and nutritional illnesses. The group of diseases and injuries with the highest burden in males was intentional and unintentional injuries [2.789 million DALYs], while in the female population this position was held by mental disorders with 1.191 million DALYs. The single most important cause of burden was traffic accidents in males and ischemic heart disease in females. Disease burden showed considerable variability between different provinces. The profile of health and disease in Iran has generally shifted from the predominance of communicable, maternal/perinatal, and nutritional illnesses towards predominance of non-communicable diseases and injuries at the national level. These figures on disease burden at population level are the most objective evidence that can be used in policy making and management of health programs, health research, and resource development within the health sector